• REVIEW: Combining CRISPR-Cas9 and Organoid Technologies to Study Gastrointestinal Diseases

REVIEW: Combining CRISPR-Cas9 and Organoid Technologies to Study Gastrointestinal Diseases

CRISPR-Cas9 technology can be used to engineer organoids for studies of gastrointestinal development and disease pathogenesis. In the February issue of Gastroenterology, Masayuki Fujii et al review derivation of organoids from human gastrointestinal tissues and how CRISPR-Cas9 has advanced studies of the digestive system. CRISPR-Cas9 genome editing technology allows researchers to

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Identification of Human Monoclonal Antibodies That Neutralize Pandemic GII.4 Noroviruses

Human monoclonal antibodies able to neutralize live human norovirus GII.4 Sydney 2012—the predominant strain responsible for recent outbreaks—are reported in the December issue of Gastroenterology. Researchers analyzed these antibodies and identified the neutralizing epitopes, providing insight into the human immune response to this deadly virus. Human noroviruses are the major

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  • Model: Microfabricated Crypt Scaffolds for Studying Human Colon Stem Cells

Model: Microfabricated Crypt Scaffolds for Studying Human Colon Stem Cells

Researchers created an in vitro human colon crypt array that provides an accurate representation of human intestinal crypts, reported in the February issue of Cellular and Molecular Gastroenterology and Hepatology. This system re-creates an intestinal epithelium with a high density of polarized crypts that respond in a physiologic manner to growth factors, metabolites, or

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  • An Ex-vivo Model for Electrolyte Transport Along the Small Intestine

An Ex-vivo Model for Electrolyte Transport Along the Small Intestine

Undifferentiated or crypt-like, and differentiated or villus-like, human intestinal enteroids represent distinct points along the crypt–villus axis and can be used to characterize electrolyte transport processes along the small intestine, researchers report in the March issue of Gastroenterology. Studies of their duodenal enteroid model showed that electrogenic Na+/HCO3– cotransporter 1 in the

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