• REVIEW: Combining CRISPR-Cas9 and Organoid Technologies to Study Gastrointestinal Diseases

REVIEW: Combining CRISPR-Cas9 and Organoid Technologies to Study Gastrointestinal Diseases

CRISPR-Cas9 technology can be used to engineer organoids for studies of gastrointestinal development and disease pathogenesis. In the February issue of Gastroenterology, Masayuki Fujii et al review derivation of organoids from human gastrointestinal tissues and how CRISPR-Cas9 has advanced studies of the digestive system. CRISPR-Cas9 genome editing technology allows researchers to

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  • Model: Microfabricated Crypt Scaffolds for Studying Human Colon Stem Cells

Model: Microfabricated Crypt Scaffolds for Studying Human Colon Stem Cells

Researchers created an in vitro human colon crypt array that provides an accurate representation of human intestinal crypts, reported in the February issue of Cellular and Molecular Gastroenterology and Hepatology. This system re-creates an intestinal epithelium with a high density of polarized crypts that respond in a physiologic manner to growth factors, metabolites, or

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In Vitro Stomach?

Researchers have developed an advanced, long-term, 3-dimensional organoid culture system for primary, untransformed human gastric epithelium. The system, described in the January issue of Gastroenterology, provides evidence for the presence of stem cells in adult human gastric tissue and can be used to study changes that occur in the gastric epithelium during

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  • How is Notch Activated in Intestinal Stem Cells?

How is Notch Activated in Intestinal Stem Cells?

The enzyme ADAM10 serves as a cellular scissors that clips and activates Notch in the intestine, required for subsequent cell lineage specification and intestinal stem cell survival, researchers report in the October issue of Gastroenterology. These Notch-activated stem cells appear to have a competitive advantage for replenishing the stem cell

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